Understand and be able to describe how translation initiation depends on a looped complex. I suggest you draw this complex out and practice labeling it.

Understand why the above contributes to translational control

Understand that transcripts can be more or less stable depending on the elements found within

Be able to describe the elements

• AREs
• Response elements (such as the iron response element)

Understand the regulation of alternate splicing and what proteins mediate this

Understand how variation in proteins can be achieved with multiple poly-A sites

For the following mechanisms, understand/describe (to the detail discussed in lecture):

• The sequence features required for the mechanism to take place
• The steps of the mechanism
• The proteins involved at each step
• The outcome(s) of the mechanism

Mechanisms:

• miRNA processing and targeting
• siRNA processing and targeting

For mechanisms as well as the other proteins mentioned in the learning objectives, think about what would happen if you removed their functionality through mutagenesis.