Understand the difference between the 43S preinitiation complex, 48S particle and the fully assembled 80S ribosome complex

Be able to list and describe the role of the eukaryotic initiation factors (eIF3, eIF1a, eIF2) and be able to compare them to the prokaryotic IFs

Understand the role of eIF5 and eIF5B Be able to list and briefly describe the proteins bound by eIF4G

Describe the Kozak sequence and how it contributes to helping the 43S particle find the right start codon

Understand the functional consequence of secondary structures in the 5' untranslated region

Understand how eukaryotic translation is regulated by phosphorylation of:

• 4E-BP
• eIF2

In a general sense, understand the steps in elongation (not to any great detail)

In a general sense, understand the process of translation termination and ribosome recycling

For the following mechanisms, understand/describe (to the detail discussed in lecture):

• The sequence features required for the mechanism to take place
• The steps of the mechanism
• The proteins involved at each step
• The outcome(s) of the mechanism

Mechanisms:

• Non-stop mediated decay (both prokaryotes and eukaryotes)
• Non-sense mediated decay (eukaryotes)

For mechanisms as well as the other proteins mentioned in the learning objectives, think about what would happen if you removed their functionality through mutagenesis.