Understand the transcriptional ground state in eukaryotes (and how this differs from prokaryotes)

Know the difference between heterochromatin and euchromatin

Understand how positive regulation of transcription occurs in eukaryotes (be able to describe the figure on slide 8 of lecture 15)

Describe in a general sense what the histone code is (no need for specific examples)

Understand the structure of an activator

• Activation domain
• DNA-binding domain including the classes

Understand the role and mechanism of an insulator and why they are important

Be able to describe what a response element is with examples (see slide 31 of lecture 15)

For the following mechanisms, understand/describe (to the detail discussed in lecture):

• The genomic features required for the mechanism to take place
• The steps of the mechanism
• The proteins involved at each step
• The outcome(s) of the mechanism

Mechanisms:

• Gene inactivation by histone deacetylase
• Gene activation by activators Including what they recruit
• Combinatorial control of transcription (using the GAL4-UAS system as a reference)

For mechanisms as well as the other proteins mentioned in the learning objectives, think about what would happen if you removed their functionality through mutagenesis.