Describe the steps and proteins involved in how a transposon creates a duplicated target sequence.

Understand the importance of the genes carried in the transposable element on how the element functions.

Understand the structure of transposons

• Insertional sequences (IS)
• Composite transposons

For the following mechanisms, understand/describe:

• The genomic features required for the mechanism to take place
• The steps of the mechanism
• The proteins involved at each step
• The outcome(s) of the mechanism

Mechanisms:

Site specific recombination

Transpositional recombination

• Cut and paste
• Replicative
• RNA-mediated
• EP retrotransposition
• TP retrotransposition

Eukaryotic transposons (specifically the Ac/Ds transposable element)

For mechanisms, think about what would happen if you removed the functionality of each of the proteins.

Assays to be familiar with:

• Cesium chloride gradient assays