Understand the different outcome of a DNA nick or double stranded break (DSB).

Describe the four possible outcomes of a damaged template on the replication fork.

For the following mechanisms, understand/describe:

• The steps of the mechanism
• The proteins involved at each step
• The outcome(s) of the mechanism

Mechanisms to repair a DSB:

• Synthesis dependant strand annealing (SDSA)
• Homologous recombination
• Non homologous end joining (NHEJ)

Other mechanisms:

• Programmed generation of DSBs during meiosis

For mechanisms, think about what would happen if you removed the functionality of each of the proteins.

Assays to be familiar with:

• Shift/gel mobility assay