Be able to describe the differences between the conservative, semiconservative and dispersive models of DNA replication (and know which one has been shown to be in use in our DNA)

Define and describe a replicon

Understand that replication happens in a bidirectional manner

Know the directionality of DNA replication

Understand the structure of the replication fork and the activities that take place

Know and describe the substrates required for DNA synthesis

Understand the concept of DNA synthesis as a processive mechanism and what contributes to that

Understand how a free 3'OH is required for synthesis

Understand the fidelity of DNA polymerase III and what contributes to the accuracy

• Shape
• Proofreading ability

Understand how the helicase activity at the replication fork alters twist ahead of the fork

Assays/techniques to be familiar with:

• Ultracentrifugation
• Genomic screening (slide 14)