Understand the uses of PCR (basically everything in molecular biology uses PCR!)

Be able to design primers to amplify a region of interest using PCR

Understand the difference between genomic DNA and cDNA

Be able to describe the differences between Sanger sequencing and next generation (flow cell/Illumina) sequencing

Be able to describe the differences between the genome and the transcriptome

Assays/techniques to be familiar with:

• PCR
• Quantitative PCR
• Reverse transcriptase PCR (RT-PCR)
• DNA fingerprinting/STR analysis
• cDNA cloning
• Sanger chain termination sequencing
• Illumina sequencing
• Microarrays (in general)
• RNA sequencing (in general)