Describe what a restriction endonuclease does and what they generate

Given a DNA sequence and a set of restriction endonucleases, be able to determine how the REs will digest the DNA

Define:

• plasmid/vector
• polylinker/multiple cloning site
• competence
• selectable marker
• genomic library and how to create one
• cDNA library and how to create one
• probe

Describe the structure of a typical plasmid/vector

Describe the different ways we can transform bacteria

Describe the different types of vector (plasmid, BAC, YAC)

Assays/techniques to be familiar with:

Cloning

• DNA isolation
• Digestion with restriction endonucleases
• Ligation
• Transformation
• Antibiotic selection
• Replica plating
• Gel electrophoresis

Restriction mapping

Probe hybridization

DNA (Southern) blots

RNA (Northern) blots

DNA fingerprinting